Goldstone Scientific Blog

Bilateral and Unilateral Fiberoptics for Head-Fixed and Non-Moving Animals

Posted by

The Prizmatix Optogenetics LED systems shown on our webstore are configured for moving animals. Many researchers use head-fixed, anesthetized, or other nonmoving animals and wonder what the set up should look like.

How to configure Bilateral fiberoptics without a rotary joint

Many customers want to deliver light bilaterally and wonder if they can connect the Y shaped fiber directly to the Optogenetics-LED and skip the rotary joint.

The answer is “No”, this may not be done.

Why? The connector of the Y shaped fiber has a lot of epoxy filling up the dead space around the two fibers. If the fiber would be attached directly to the LED, the epoxy would melt and damage the fiberoptic and the LED. It is necessary to have a wide core mediating fiber between the LED and the Y shaped bundle. The wide core fiber delivers the light to the two branches.

But not to worry. You don’t need to buy a rotary joint. A FC-FCstationary mating connector can be used instead of the rotary joint. This will reduce the cost of the fiber assembly significantly.

The parts needed are:

Note: When using the FC-LED series which has lower power than our Optogenetics-LED, the Y shaped fiber can be connected directly to the LED without a mediating fiber. In that case, Prizmatix can make a custom fiberoptic with an SMA connector.

How to configure Unilateral fiberoptics without a rotary joint

When the animal won’t be moving and the stimulation is needed in only one implanted fiber, the fiber optics can go directly from the LED to the animal. But the usual “RJ to animal” fiber can not be used. Those fibers have an FC connector, and the LED has an SMA connector. So, the fiber needs a SMA connector on the LED side and a 1.25 or 2.5mm ferrule on the animal side. This special fiber available on the webstore here.

Please contact us if you have any question about how to configure your fiber optics.

View Comments

How to make your own fiberoptic implants for Optogenetics

Goldstone Scientific offers the supplies you need to prepare your own fiber optic implants (“cannulae”) for In-Vivo Optogenetic experiments. Prizmatix created this step-by-step video tutorial which shows you how to do it. Written Instructions can be seen at: The products used in the video are: DIY Kits, includes almost everything to get started including epoxy. Does not include magnifier.Raw Fiber Empty FerrulesAdditional supplies [...]

Read More »

Illumination Inside of an Incubators and CO2 Chambers – Part II

I wrote the  first blog post on the topic on illumination inside of an incubator, in 2014. There have been major changes since then. The challenge has not changed; the solutions have. The challenge is to deliver intense light over a microwell plates or petri dishes inside of a chamber where the conditions could damage the LED, and [...]

Read More »

Light Meters, Fiber Optics and Optogenetics – Part 5 Little Mistake, Big Problem

A customer was getting much lower power than expected from the fiber optics of his Prizmatix Optogenetics LED. His entire system including his power meter was shipped to our lab for debugging. Turns out the whole problem was the FC adapter on the detector of his power meter. The purpose of this FC fiberoptic connector/adapter is to [...]

Read More »

Light Meters, Fiber Optics and Optogenetics – Part 4 –Using Integrating Sphere Correctly

In my  previous blog post about Light Meters I explained why the ideal detector for measuring light from a LED compatible high NA fiber optic is a detector built into an integrating sphere. This post describes the best method for taking the measurements from Prizmatix fiber optic experts. Here is their advice: The correct way of measuring Power for [...]

Read More »

Light Meters, Fiber Optics and Optogenetics – Part 3 – Prizmatix’s Advice on Light Meters for Optogenetics

I am often asked to recommend light meters to use for testing the fiber optic implants that will be used in Optogenetic experiments. This is important in order to test the quality of each implant and make sure everything is working correctly. Measuring the light correctly is not a trivial matter. The meter setting, the detector and [...]

Read More »

High NA Fiber Optics for In-Vivo Optogenetics – The Options on Goldstone Scientific

Quality fiber optic implants are crucial for successful in-vivo Optogenetics. Scientists will need to spend time or money (or both) to ensure quality results. Prizmatix offers a range of options and Goldstone Scientific makes it easy to order and offers competitive prices with bulk discounts and fast shipping to save you both. Many items are [...]

Read More »

Light Meters, Fiber Optics and Optogenetics – Part 1 – Light Meter Blues

Prizmatix LEDs are extremely powerful and our customers are generally very satisfied with their performance.  Sometime, however, I get complaints about the power output being lower than expected as per the published specification. Very frequently it turns out that the light source is fine, and the problem lies within the tools and methods used to measure the power. In [...]

Read More »

Prizmatix Fiber Optic Rotary Joint; Compatibility of with Lasers or other light sources

The Prizmatix Fiber Optic Rotary Joint is an important part of the Prizmatix  In-Vivo Optognetics Toolbox. It’s extremely low torque (<10μNm) allows even the smallest animals to move freely and its high transmission is helpful in getting as much power as possible to the target. One really nice feature of the Prizmatix fiber optic rotary joint is that it can be used [...]

Read More »

Do LEDs have enough power for In-Vivo Optogenetics?

Do LEDs have enough power for In-Vivo Optogenetics?  It depends who you ask. Often, while talking to researchers considering LED for Optogenetics, I hear “People tell me that LEDs just don’t have enough power, especially for inhibition”. That is a real concern. In fact a competing vendor of LEDs for Optogenetics told me that their light sources do not have enough power, [...]

Read More »